Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen.

Phytic Acid Metabolism in Lily (Lilium longiflorum Thunb.) Pollen.

The accumulation of phytic acid during development of lily (Lilium longiflorum Thunb.) pollen and its degradation during germination have been studied. A substantial amount of phytic acid accumulates in lily pollen by 5 days before anthesis, and little change occurs during subsequent maturation. Mature lily pollen contains 7 to 8 micrograms phytic acid per milligram pollen. Considerable degrada...

Unique alkaline phytase from lily pollen: cloning, characterization and differential expression

Purification and characterization of an acidic, thermophilic phytase from a newly isolated Geobacillus stearothermophilus strain DM12

Microbial phytases were applied mainly to animal and human foodstuffs in order to improvemineral bioavailability and food processing. In addition, phytases have potentialbiotechnological application in various other fields, such as environmental protection,aquaculture and agriculture. Bacillus sp. DM12, an isolate from a hot spring, produces phytase,which catalyzes the hydrolysis of phytic acid...

The effect of minerals and mineral chelators on the formation of phytase-resistant and phytase-susceptible forms of phytic acid in solution and in a slurry of canola meal

Minerals can readily bind to phytic acid and thus have the potential to form mineral±phytate complexes that may be resistant to hydrolysis by phytase activity of animal, plant and microbial origin. In simple solution, at pH 7.0, mineral concentrations from 0.053 mM for Zn up to 4.87 mM for Mg caused a 50% inhibition of phytate-P hydrolysis by microbial phytase. The rank order of mineral potency...

A Calcium-Activated Phytase from Pollen of Lilium longiflorum.

A phytase was isolated and partially purified from the pollen of Lilium longiflorum Thumb. Optimum activity was at pH 8.0. The phytase was activated by Ca(2+) and Sr(2+) but not by the other divalent cations tested. Activity was inhibited by ethylenediaminetetraacetate. The phytase had a temperature optimum of 55 to 60 degrees C and an activation energy of about 12,700 calories/mole. Extraction...